Composite

Part:BBa_K2627006:Design

Designed by: Jiao Jin   Group: iGEM18_SDU-CHINA   (2018-10-10)


paraBAD-crRNA targeting gltA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 253
    Illegal XhoI site found at 365
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 88
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 70


Design Notes

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Source

iGEM18_SDU-CHINA

References

Chang Y, Su T, Qi Q*, Liang Q*. Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system. Microb Cell Fact. 2016 Nov 15;15(1):195...